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Journal of Exercise Nutrition & Biochemistry 2011;15(3):124-130.
The effect of puer-tea supplementation on antioxidant enzymes during acute exercise of rats fed a high fat diet
Abstract
This study purposed to examine the effect of puer-tea supplementation on antioxidant enzymes during acute exercise of rats. Thirty-two rats were randomly divided into four groups (HS: high fat diet sedentary group, n=8; HE: high fat diet acute exercise group, n=8; PS: puer-tea gavaged sedentary group, n=8; puer-tea gavaged acute exercise group, n=8), The high-fat diet was composed of 35% carbohydrate, 20% protein, and 35% fat. In the acute exercise group, the rats were swam for 60min (3% load/weight) before anesthetized with ether. The results showed that EFT of PS group was significanlty lower than the HS group (p<.05). In the blood components, there were no significantly different in the serum glucose, TC, HDLC, but TG level of exercise group were significantly lower than the control group (p<.01), respectively. In addition muscle glycogen content of PS and PE were significantly higher than the HS and HE (p<.01), respectively, and MDA content of PE was lower than the PS group (p<.01). Furthermore, Cu,Zn-SOD protein expression of PS group was significantly higher than the HS group (p<.05), and GPx protein expression of PE and HE were significantly higher than the PS and HS (p<.01), respectively. From these results, puer-tea supplementation has a positive effect on antioxidant activity because of puer-tea supplemented group was showed higher value of Cu,Zn-SOD protein expression, and could be an important dietary antioxidant source as well.
Key Words: puer-tea, antioxidant enzymes, acute exercise, Cu,Zn-SOD, GPx


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