The effects of a acute endurance exercise in hot emvironment on HSP70 and Small HSP expression of skeletal muscle in rats |
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Abstract |
The purpose of this study was to determine to the expression of a acute endurance exercise in hot ambient temperatures on the skeletal muscle. Specifically, this study investigates the effects of hot temperature to the heat shock protein 70, heat shock protein 27 and aB-crystallin, Creatine kinase, blood lactate, plasma CK during exercise in the rats. Adult male Sprague-Dawley rats were randomly placed in one of two ambient temperature groups (normal, hot). The treadmill running intensity is 27 m/min, 10o intensity and soleus and extensor digitorum longus muscle was extracted at 30, 60, 90 min in exercise period. The expression of HSP70, HSP27, aB-crystallin were analyzed by western blotting. The level of small HSP was significantly increased in hot and normal ambient temperature. But the level HSP70 was significantly increased in hot ambient temperatures only. These results suggesting that increased apoptotic factor by hot ambient temperature may causes the expression of HSP70 and small HSP may involved cell damage in early stage of exercise not ambient temperature. |
Key Words:
HSP70, Small HSP, aB-crystallin, Creatine kinase, EDL, Soleus, Apoptotic |
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